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Construction and use of a dialysis chamber for investigating in situ the toxicity of heavy metals on bacteria. ArchiMer
Tan, T.
Teflon dialysis sampling chambers have been employed to study the toxicity of ZnCl sub(2) on a cadmium-sensitive bacterium, Acinetobacter) CS 13, in an aquarium with running seawater. A concentration of 10 mg Zn super(++) l super(-1) did not influence the cell densities in the chambers, but cell densities decreased at zinc concentrations of 15 and 20 mg l super(-1). In seawater with 20 mg Zn super(++) lu-1 the bacteria accumulated zinc from 827 to 2813 mu g g super(-1) cells dry wt after 1 d. After 2 d the zinc concentration increased to 4625 mu g g super(-1) dry wt. Three chambers were filled with Acinetobacter CS 13 cells added to sterile sediment at a concentration of 1 x 10 super(8) cells ml super(-1) wet sediment. To test the security, these chambers...
Tipo: Text Palavras-chave: Acinetobacter; Bacteria; Laboratory culture; Tests; Zinc; Dialysis; Toxicity; Heavy metals.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1012.pdf
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